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Best Practices

Stool Culture

Recommendations document to public health laboratories for the isolation and characterization of STEC from Shiga toxin-positive stools or specimens posted.

Last Updated on Friday, 20 September 2013 13:19

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Medical Microbiology Questions

Medical Microbiology Questions
This "Question of the Day" listserv originated out of an effort to aid clinical microbiology fellows in their preparation for the American Board of Medical Microbiology (ABMM) board exam. The list of subscribers has since evolved beyond microbiology fellows to include pathology residents and fellows, infectious disease fellows and faculty members in various disciplines. Questions are designed to address all areas of medical microbiology with the hope that they will provide continuing education and support board preparation, be it the ABMM, clinical pathology or infectious diseases.
This listserv is not endorsed or supported by the ABMM.
www.pathquestions.com/micro

Last Updated on Friday, 09 May 2014 15:51

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Q-PROBES

A CAP Quality Assurance Program, Q-PROBES provide a one-time comprehensive assessment of key laboratory processes. Q-PROBES help build and improve data collection and analysis processes that contribute to quality of care, patient safety and outcomes.
http://tinyurl.com/btxyb7z

Last Updated on Friday, 09 May 2014 10:16

Hits: 3115

Help with Parasites

Advances in Laboratory Detection of Trichomonas vaginalis:  
An APHL document that gives a brief overview of clinical information, epidemiology and reviews diagnostics options for T. vaginalis.

Last Updated on Friday, 09 May 2014 15:52

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Help with Fungi

The following helpful tables are from the Manual of Clinical Microbiology, 10th ed. (J. Versalovic, K.C. Carroll, G. Funke, J.H. Jorgensen, M.L. Landry, and D.W. Warnock, ed.; ASM Press, Washington, DC, 2011), ASMscience
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 Methods and Stains for Direct Microscopic Exam of Fungi
A number of stains or procedures can be used to detect fungal organisms by direct examination of clinical specimens.

 Selection of Clinical Specimens for Opportunistic Fungal Pathogens
Certain fungal organisms are more likely to be recovered from specific body sites.

From Y. R. Shea, “General Approaches for Direct Detection of Fungi,” chapter 114, p. 1776–1792, in J. Versalovic, K. C. Carroll, G. Funke, J. H. Jorgensen, M. L. Landry, and D. W. Warnock (ed.), Manual of Clinical Microbiology, 10th ed., vol. 2 (© ASM Press, Washington, DC, 2011).

Purchase
Visit ASMscience to purchase MCM10 in print and/or electronic format.

ASM's Quick Guide to the Significance and Laboratory Identification of Cryptococcus gattii
C. gattii is an emerging agent of cryptococcosis that is indistinguishable from C. neoformans by most routine laboratory tests.

Mycology Terms
The University of South Carolina School of Medicine's medical mycology glossary.
http://www.microbiologybook.org/mycology/glossary.htm

University of Adelaide's Mycology Website
This website contains information on the identification and management human and animal fungal infections.  It provides a range of educational materials including a mold identification self assessment module, descriptions of fungal pathogens and diseases, and links to societies and to other mycology sites.
http://www.mycology.adelaide.edu.au/

 
memonly-sm For Members

Last Updated on Wednesday, 02 July 2014 15:12

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Help with Viruses

 

Last Updated on Friday, 04 April 2014 12:49

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Help with Bacteria

Transportation of Specimens for Neisseria gonorrhoeae Culture     
This APHL document describes the systems suitable for transporting N. gonorrhoeae specimens. It provides available performance data, and compares the benefits and limitations of each system.

Last Updated on Thursday, 29 May 2014 11:40

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Gram Stain Tutorial

Gram Stain Tutorial
This MicrobeLibrary "animated" PowerPoint presentation provides:
1. An introduction (brief history and structural basis) to the Gram Stain.
2. Stepwise instructions for the techniques.
3. "Cartoons" of how the cells appear at each step.
4. Views of typical Gram morphologies.

 

Last Updated on Monday, 19 December 2011 07:00

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Mycobacteriology

Mycobacterial Cultures
The CDC's Division of Tuberculosis Elimination (DTBE) Laboratory Branch provides services tests on mycobacterial cultures. Any local health department, licensed physician's office, licensed laboratory or licensed health care facility may submit cultures for testing but they must be routed through either their state health department or other authorized facility.
http://www.cdc.gov/tb/topic/laboratory/default.htm

Molecular Epidemiology of Tuberculosis: Current Insights
A 2006 Clinical Microbiology Reviews' article that reviews the current methods, concepts, and applications of molecular approaches used to better understand the epidemiology of TB.
B. Mathema, et al. October 2006 19: 46-65
Abstract and PDF
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Method for Inactivating and Fixing Unstained Smear Preparations of Mycobacterimum tuberculosis for Improved Laboratory Safety
This 2002 Journal of Clinical Microbiology abstract states: The inactivation of smears that contain Mycobacterium tuberculosis for microscopy before removal of the material from a biosafety cabinet is an important safety factor in preventing the potential transmission of tuberculosis to laboratory workers. The fixing and inactivating properties of heat flaming, 70% ethanol, and 1, 3, and 5% phenol in ethanol for smears containing M. tuberculosis were investigated. Heat flaming failed to inactivate the smear material, whereas 5% phenol in ethanol successfully fixed and inactivated all smears containing M. tuberculosis both from concentrated sputum samples and from culture material.
P. Chedore, et al.   
J. Clin. Microbiol. November 2002 40:4077–4080.
Abstract and PDF
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TB Self-Assessment Tool

Developed by the CDC and the Association of Public Health Laboratories, this tool is designed to assist laboratories in the assessment of the quality of their laboratory's TB diagnostic practices.
http://www.aphl.org/aphlprograms/infectious/tuberculosis/Pages/TB-Self-Assessment-Tool.aspx

CDC's Reference for TB Testing and Diagnosis
There are two kinds of tests that can be used to help detect TB infection – the TB skin test (TST) and TB blood tests. 
http://www.cdc.gov/tb/topic/testing/default.htm

Last Updated on Friday, 09 May 2014 15:54

Hits: 3879

Blood Cultures

The CDC launched a new website on sepsis in the hopes to increase awareness and improve recognition, diagnosis, and treatment of patients. 

Last Updated on Monday, 09 June 2014 14:20

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Respiratory Cultures

Ventilator-Associated Pneumonia: Diagnosis, Treatment, and Prevention
This 2006 Clinical Microbiology Reviews' article reviews the literature with regard to diagnosis, treatment, and prevention. It provides conclusions that can be implemented in practice as well as an algorithm for the bedside clinicial and also focuses on the controversies with regard to diagnostic tools and appraoches, treatment plans, and prevention strategies.
S.M. Koenig and J.D. Truwit
Clin. Microbiol. Rev. October 2006 19: 637-657
Abstract and PDF
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Last Updated on Tuesday, 19 November 2013 09:34

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