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Help with Viruses

 
HAN 369: Severe Respiratory Illness Associated with Enterovirus D68
A CDC HAN Advisory explaining EV-D68 as a possible cause of acute unexplained respiratory illness.
http://emergency.cdc.gov/HAN/han00369.asp
 
 
HCV Guidelines
HCV's website is designed to help physicians treat hepatitis C infection.
http://hcvguidelines.org/
 
The Big Picture Book of Viruses
This site is intended to serve as both an Internet catalog of virus pictures and as an educational resource to those seeking more information about viruses.
http://www.virology.net/Big_Virology/BVHomePage.html
 
The following helpful tables are from the Manual of Clinical Microbiology, 10th ed. (J. Versalovic, K.C. Carroll, G. Funke, J.H. Jorgensen, M.L. Landry, and D.W. Warnock, ed.; ASM Press, Washington, DC, 2011), ASMscience
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HIV antibody testing algorithm for samples tested with rapid HIV assays. LIA, line immunoassay.

From B. P. Griffith, S. Campbell, and A. M. Caliendo, “Human Immunodeficiency Viruses,” chapter 79, p. 1302–1322, in J. Versalovic, K. C. Carroll, G. Funke, J. H. Jorgensen, M. L. Landry, and D. W. Warnock (ed.), Manual of Clinical Microbiology, 10th ed., vol. 2 (© ASM Press, Washington, DC, 2011).
 
Algorithm for HIV Confirmatory Tests
HIV antibody testing algorithm for samples initially reactive in a standard HIV-1/HIV-2assay showing the sequence of suggested follow-up confirmatory testing. LIA, line immunoassay.
 
From B. P. Griffith, S. Campbell, and A. M. Caliendo, “Human Immunodeficiency Viruses,” chapter 79, p. 1302–1322, in J. Versalovic, K. C. Carroll, G. Funke, J. H. Jorgensen, M. L. Landry, and D. W. Warnock (ed.), Manual of Clinical Microbiology, 10th ed., vol. 2 (© ASM Press, Washington, DC, 2011).
 
eMethods for Detection and Identification of Viruses
A concise overview of the methods available for detection and identification of viruses differ and continue to evolve. 
 
From M. L. Landry, A. M. Caliendo, C. C. Ginocchio, Y.-W. Tang, and A. Valsamakis, “Algorithms for Detection and Identification of Viruses,” chapter 78, p. 1297–1301, in J. Versalovic, K. C. Carroll, G. Funke, J. H. Jorgensen, M. L. Landry, and D. W. Warnock (ed.), Manual of Clinical Microbiology, 10th ed., vol. 2 (© ASM Press, Washington, DC, 2011). 

Methods used for Detection of Viruses
There have been many advances in diagnostic virology and the test(s) of choice vary by virus and syndrome. 
 
From M. S. Forman and A. Valsamakis, “Specimen Collection, Transport, and Processing: Virology,” chapter 76, p. 1276–1288, in J. Versalovic, K. C. Carroll, G. Funke, J. H. Jorgensen, M. L. Landry, and D. W. Warnock (ed.), Manual of Clinical Microbiology, 10th ed., vol. 2 (© ASM Press, Washington, DC, 2011). 
 
Molecular Tests for Flu
Commercially available and selected other molecular detection assays for influenza viruses.
 
From R. L. Atmar and S. E. Lindstrom, “Influenza Viruses,” chapter 81, p. 1333–1346, in J. Versalovic, K. C. Carroll, G. Funke, J. H. Jorgensen, M. L. Landry, and D. W. Warnock (ed.), Manual of Clinical Microbiology, 10th ed., vol. 2 (© ASM Press, Washington, DC, 2011). 

Rapid and Point of Care HIV Tests 
Six rapid HIV antibody tests were FDA approved in the U.S. as of July 2009. 
 
From B. P. Griffith, S. Campbell, and A. M. Caliendo, “Human Immunodeficiency Viruses,” chapter 79, p. 1302–1322, in J. Versalovic, K. C. Carroll, G. Funke, J. H. Jorgensen, M. L. Landry, and D. W. Warnock (ed.), Manual of Clinical Microbiology, 10th ed., vol. 2 (© ASM Press, Washington, DC, 2011).
 
Rapid Influenza Tests
A number of commercially available kits utilize the immunoassay format for rapid (?30-min) detection of influenza A and B viruses in clinical specimens.
 
From R. L. Atmar and S. E. Lindstrom, “Influenza Viruses,” chapter 81, p. 1333–1346, in J. Versalovic, K. C. Carroll, G. Funke, J. H. Jorgensen, M. L. Landry, and D. W. Warnock (ed.), Manual of Clinical Microbiology, 10th ed., vol. 2 (© ASM Press, Washington, DC, 2011). 
 
RSV and MPV Commercial Tests
Respiratory Syncytial Virus and Human Metapneumovirus commerical tests.
 
From Y.-W. Tang and J. E. Crowe, Jr., “Respiratory Syncytial Virus and Human Metapneumovirus,” chapter 83, p. 1357–1371, in J. Versalovic, K. C. Carroll, G. Funke, J. H. Jorgensen, M. L. Landry, and D. W. Warnock (ed.), Manual of Clinical Microbiology, 10th ed., vol. 2 (© ASM Press, Washington, DC, 2011). 
 
RSV Rapid Antigen Tests
In addition to immunofluorescent assay (IFA), there are several rapid antigen assays are commercially available
 
From Y.-W. Tang and J. E. Crowe, Jr., “Respiratory Syncytial Virus and Human Metapneumovirus,” chapter 83, p. 1357–1371, in J. Versalovic, K. C. Carroll, G. Funke, J. H. Jorgensen, M. L. Landry, and D. W. Warnock (ed.), Manual of Clinical Microbiology, 10th ed., vol. 2 (© ASM Press, Washington, DC, 2011).
 
Specimens for Virus Detection
Different clinical specimens are suitable for the diagnosis of specific diseases through virologic testing. 
 
From M. S. Forman and A. Valsamakis, “Specimen Collection, Transport, and Processing: Virology,” chapter 76, p. 1276–1288, in J. Versalovic, K. C. Carroll, G. Funke, J. H. Jorgensen, M. L. Landry, and D. W. Warnock (ed.), Manual of Clinical Microbiology, 10th ed., vol. 2 (© ASM Press, Washington, DC, 2011). 
 
Purchase
Visit ASMscience to purchase MCM10 in print and/or electronic format.

CDC Flu
The CDC updates you on what to do about the flu.
http://www.flu.gov/

Middle East Respiratory Syndrome
This CDC website provides guidelines for laboratories, health departments, and healthcare providers.
http://www.cdc.gov/coronavirus/mers/index.html

Evaluation of Rapid Influenza Diagnostic Tests for Influenza A (H3N2)v Virus and Updated Case Count
This Morbidity and Mortality Weekly Report (MMWR), Early Release provides 1) an update on the number of cases of H3N2v infections in the United States, 2) an updated results interpretation for the CDC rRT-PCR Dx Panel for A(H3N2)v for public health laboratories, and 3) an evaluation of rapid influenza diagnostic tests for the detection of H3N2v viruses.
http://www.cdc.gov/mmwr/pdf/wk/mm61e0810.pdf

Rapid Diagnostic Testing for Influenza
A CDC guidance document on influenza rapid diagnostic tests.
http://www.cdc.gov/flu/professionals/diagnosis/rapidlab.htm

Updated Norovirus Outbreak Management and Disease Prevention Guidelines
A Morbidity and Mortality Weekly Report, it reviews recent advances and provides guidelines for outbreak management and disease prevention. These recommendations are intended for use by public health professionals investigating outbreaks of acute gastroenteritis, including state and local health authorities, as well as academic and research institutions.
http://www.cdc.gov/mmwr/preview/mmwrhtml/rr6003a1.htm?s_cid=rr6003a1_e

WHO's Measles Lab Manual
This manual provides information about the World Health Organization Laboratory Network for Measles and Rubella and detailed protocols for cell culture, virus isolation, collection and handling of specimens for the isolation and identification of measles virus. There is also information on biosafety and international shipping regulations. These techniques have been used successfully at the Centers for Disease Control and Prevention for investigation of measles outbreaks.
http://www.cdc.gov/measles/lab-tools/WHO-lab-manual.html

 
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